Technology

EpiSwitch™ is a proprietary industrial platform for the discovery, evaluation, validation and monitoring of a novel class of epigenetic biomarkers known as ‘chromosome conformation signatures’ (“CCSs”). CCSs can provide a compelling, stable framework from which changes in the regulation of a genome can be analysed, long before the results of these epigenetic changes manifest themselves as obvious abnormalities.

Chromosomal Conformation Capture

The packaging of chromosomal DNA plays a critical role in the epigenetic regulation of the whole genome. It ensures effective storage, access to genetic information and its regulation by the complex protein machinery utilised in gene expression. Known also as ‘gene loops’, ‘long-range chromosomal interactions’ and ‘chromatin domains’, CCSs have been recognised as an essential high-level framework of epigenetic regulation imposed across the whole genome and reflecting its fractal nature of organisation.

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Episwitch™ Pipeline

STAGE 1 – DESIGN

Episwitch™ Design Annotation

Proprietary pattern recognition bioinformatic algorithms used to detect specific DNA sites as high probability targets for CCS detection

STAGE 2 – DISCOVER

Episwitch™ Array

High throughput screening and statistical analysis performed to detect high-order chromosomal structures (i.e. a short list of CCS biomarker candidates for further analysis).

Episwitch™ PCR Platform

Translation and verification of selected marker candidates for further reduction and cross-validation of stratifying signatures, ready for validation.

Episwitch™ NGS

High throughput sequencing and bioinformatic analysis to detect high-order chromosomal conformation signatures, ready for cross-validation by other modalities.

STAGE 3 – VALIDATE

Episwitch™ PCR Platform

Validation of the Selected marker signatures on independent sample cohorts for use by OBD’s commercial partners.

Our Publications on Episwitch™


Super-Enhancers and Broad H3K4me3 Domains Form Complex Gene Regulatory Circuits Involving Chromatin Interactions.

Scientific Reports 7, Article number: 2186; 2017 May 19

https://www.nature.com/articles/s41598-017-02257-3

Chromatin Conformation Signatures Associated with Epigenetic Deregulation of the FIP1L1 and PDGFRA Genes.

American Society of Haematology, 58th Annual Meeting and Exposition; 2016 Dec 3 - 6; San Diego, CA, USA

https://ash.confex.com/ash/2016/webprogram/Paper90884.html

Epigenetic footprints for neurodegenerative and autoimmune conditions: a comparative analysis.

Preclinical neurodegenerative disease: towards prevention and early diagnosis; The Lancet Neurology Conference; 2016 Oct 19 -21; London, United Kingdom

http://www.oxfordbiodynamics.com/wp-content/uploads/2016/10/2016-LNC-Epigenetic-footprints-for-neurodegenerative-and-autoimmune-conditions.pdf

Epigenetic signatures and early detection of neurodegenerative diseases.

Preclinical neurodegenerative disease: towards prevention and early diagnosis; The Lancet Neurology Conference; 2016 Oct 19 -21; London, United Kingdom

http://www.oxfordbiodynamics.com/wp-content/uploads/2016/10/2016-LNC-Epigenetic-signatures-and-early-detection-of-neurodegenerative-diseases.pdf